Phytosulphokine- , a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot
نویسندگان
چکیده
منابع مشابه
Synchronization of somatic embryogenesis in a carrot cell suspension culture.
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...
متن کاملDifferential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis.
The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-met...
متن کاملThe effects of calcium and magnesium on carrot (Dacus carrota cv. Nants) petiole somatic embryogenesis
The present study was conducted in two different experiments based on complete randomized design to evaluate the effects of calcium (0, 1, 2, 4 mM) and magnesium (0, 1.02., 2.04, 4.08 mM) on carrot petiole somatic embryogenesis in B5 medium. A difference on somatic embryogenesis was observed among different levels of calcium and magnesium. The number of globular embryos in medium with low magne...
متن کاملSomatic Embryogenesis and Plant Regeneration in Cucumber
The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars was examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and ‘Fushinarimidori’ produced the highest number of embryos from either cotyledons or young first leaves. Cotyledonand first-leaf-derived calluses pro...
متن کاملIdentification of a transitional cell state in the developmental pathway to carrot somatic embryogenesis
We have located a novel carbohydrate epitope in the cell walls of certain single cells in embryogenic, but not in non-embryogenic, suspension cultures of carrot. Expression of this epitope, recognized by the mAb JIM8, is regulated during initiation, proliferation, and prolonged growth of suspension cultures such that changes in the abundance of JIM8-reactive cells always precede equivalent chan...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 1999
ISSN: 0022-0957,1460-2431
DOI: 10.1093/jxb/50.336.1123